Abstract
Vaccination of dogs, the domestic reservoir of Leishmania chagasi, could not only decrease the burden of canine visceral leishmaniasis (VL), but could also indirectly reduce the incidence of human VL. Intramuscular vaccination of foxhounds with a Leishmania multicomponent (10 antigen) DNA vaccine resulted in antigen-induced lymphoproliferative and IFN-gamma (but not IL-4) responses. This response was not augmented by co-administration of canine IL-12 or GM-CSF DNA adjuvants. The multicomponent DNA vaccine also induced a delayed type hypersensitivity (DTH) response to viable L. donovani promastigotes and led to a reduction of parasite burden in an in vitro intracellular infection model, and in the draining lymph node of dogs early after cutaneous challenge. Thus, the multicomponent DNA vaccine was effective in priming dogs for a parasite-specific type 1 cellular immune response, which was able to restrict parasite growth.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Animals
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Antibodies, Protozoan / blood
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology
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DNA, Protozoan / analysis
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Disease Reservoirs
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Dog Diseases / prevention & control*
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Dogs
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Granulocyte-Macrophage Colony-Stimulating Factor / immunology
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Hypersensitivity, Delayed
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Interferon-gamma / biosynthesis
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Interleukin-12 / genetics
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Interleukin-12 / immunology
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Interleukin-4 / biosynthesis
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Leishmania infantum / genetics
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Leishmania infantum / growth & development
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Leishmania infantum / immunology*
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Leishmaniasis, Visceral / prevention & control
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Leishmaniasis, Visceral / veterinary*
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Lymph Nodes / parasitology
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Lymphocyte Activation
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Polymerase Chain Reaction
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Protozoan Vaccines / administration & dosage
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Protozoan Vaccines / genetics
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Protozoan Vaccines / immunology*
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RNA, Messenger / analysis
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T-Lymphocytes / immunology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Protozoan
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Antigens, Protozoan
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DNA, Protozoan
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Protozoan Vaccines
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RNA, Messenger
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Vaccines, DNA
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Interleukin-12
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Interleukin-4
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor