[A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro]

Zhonghua Gan Zang Bing Za Zhi. 2005 Nov;13(11):808-10.
[Article in Chinese]

Abstract

Objective: To synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.

Methods: HPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.

Results: The construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.

Conclusion: In vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.

Publication types

  • English Abstract

MeSH terms

  • DNA-Directed DNA Polymerase
  • DNA-Directed RNA Polymerases*
  • Hepatitis B virus / genetics*
  • RNA Processing, Post-Transcriptional*
  • RNA Splicing
  • RNA-Binding Proteins / physiology
  • Transcription, Genetic*
  • Viral Proteins*

Substances

  • RNA-Binding Proteins
  • Viral Proteins
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases
  • DNA-Directed DNA Polymerase