Abstract
A "column-format" preparative electrophoresis device which obviates the need for gel extraction or secondary electro-elution steps is described. Separated biomolecules are continuously detected and eluted directly into a minimal volume of free solution for subsequent use. An optical fiber allows the species of interest to be detected just prior to elution from the gel column, and a small collection volume is created by addition of an ion-exchange membrane near the end of the column.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Chromatography, Ion Exchange / instrumentation*
-
Chromatography, Ion Exchange / methods
-
Electrophoresis / instrumentation*
-
Electrophoresis / methods
-
Equipment Design
-
Equipment Failure Analysis
-
Escherichia coli / genetics*
-
Fiber Optic Technology / instrumentation*
-
Fiber Optic Technology / methods
-
Microchemistry / instrumentation*
-
Microchemistry / methods
-
Online Systems
-
Optical Fibers
-
RNA, Bacterial / analysis*
-
Reproducibility of Results
-
Sensitivity and Specificity
-
Specimen Handling / instrumentation*
-
Specimen Handling / methods