The effect of hyaluronan on tendon healing in rabbits

Arthroscopy. 2005 Nov;21(11):1330-6. doi: 10.1016/j.arthro.2005.08.020.

Abstract

Purpose: Enhancement of the healing of the tendon-bone junction is important for anterior cruciate ligament reconstruction using the hamstring tendon. We examined the effect of hyaluronan injection into the bone tunnel on the healing of the tendon-bone junction.

Type of study: Experimental study.

Methods: The medial half of the patella tendon of Japanese white rabbits was detached from the tibial insertion and transplanted into a drill hole in the tibial tuberosity with a pullout fixation. The bone tunnel was filled with 0.5 mL of hyaluronan (MW, 0.8 million) or without hyaluronan in the contralateral knee as the control. Biomechanical testing was performed at 3 (n = 6) and 6 weeks (n = 4) with histologic analysis (n = 3).

Results: Maximum failure load of the patella-patellar tendon-tibia complex in the knee treated with hyaluronan was higher than that in the control side in each rabbit at 3 weeks: 98 +/- 6 N in the hyaluronan group and 76 +/- 12 N in the control group (P < .01). Histologic analysis showed that hyaluronan improved tendon healing. Although maximum failure load increased in both groups, the failure load was not significant at 6 weeks.

Conclusions: Our study found improved tendon healing to bone in the first 3 weeks for Japanese white rabbits treated with hyaluronan.

Clinical relevance: Because hyaluronan treatment produces a clinical effect, even in only the early phase after ligament surgery, it could improve rehabilitation and produce a better outcome for ligament reconstruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Cell Division / drug effects
  • Hyaluronic Acid / administration & dosage
  • Hyaluronic Acid / pharmacology
  • Hyaluronic Acid / therapeutic use*
  • Injections, Intra-Articular
  • Patellar Ligament / surgery*
  • Patellar Ligament / ultrastructure
  • Rabbits
  • Stromal Cells / cytology
  • Stromal Cells / drug effects
  • Tendon Transfer*
  • Tibia / surgery
  • Wound Healing / drug effects*

Substances

  • Hyaluronic Acid