Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species

Cristinel Sandu; Calin-Bogdan Chiribau; Paula Sachelaru; Roderich Brandsch

doi:10.1128/AEM.71.12.8920-8924.2005

Plasmids for nicotine-dependent and -independent gene expression in Arthrobacter nicotinovorans and other arthrobacter species

Appl Environ Microbiol. 2005 Dec;71(12):8920-4. doi: 10.1128/AEM.71.12.8920-8924.2005.

Authors

Cristinel Sandu¹, Calin-Bogdan Chiribau, Paula Sachelaru, Roderich Brandsch

Affiliation

¹ Institute of Biochemistry and Molecular Biology, University of Freiburg, Hermann Herder Str. 7, 79104 Freiburg, Germany. [email protected]

Abstract

The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow the production of complex enzymes and genetic complementation studies in Arthrobacter species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arthrobacter / classification
Arthrobacter / genetics*
Base Sequence
Gene Expression Regulation, Bacterial / drug effects*
Genes, Reporter
Green Fluorescent Proteins / genetics
Molecular Sequence Data
Nicotine / pharmacology*
Plasmids / drug effects
Plasmids / genetics*
Restriction Mapping

Substances

Green Fluorescent Proteins
Nicotine

Associated data

GENBANK/DQ191047
GENBANK/DQ191048