Abstract
Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.
MeSH terms
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Adenovirus Infections, Human / virology*
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Adenoviruses, Human / classification*
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Adenoviruses, Human / genetics
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Adenoviruses, Human / isolation & purification
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Adolescent
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Adult
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Capsid Proteins / genetics*
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Child
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Child, Preschool
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DNA, Viral / analysis
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Genome, Viral
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Humans
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Infant
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Infant, Newborn
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Middle Aged
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA*
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Serotyping
Substances
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Capsid Proteins
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DNA, Viral
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hexon capsid protein, Adenovirus