Objective: To investigate the influence of TPA treatment on HEp-2 cell line in vitro.
Method: HEp-2 cell line cultured in vitro was treated by different concentration levels of TPA. MTT method was used to detect survival cell ratio of HEp-2.
Result: Cell growth of HEp-2 after treatment of TPA manifested an peak. The highest peak which was 1.3 to 1.4 times of control cell presented on 10 microg/L concentration level. Cell proliferation ability dropped gradually following increase of TPA concentration.
Conclusions: TPA can increase cell proliferation of HEp-2 cell in vitro.