Abstract
11Beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1) is a NADP(H)-dependent oxidoreductase of the ER lumen, which may have an important role in the pathogenesis of metabolic syndrome. Here, the functional coupling of 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase (H6PDH) was investigated in rat liver microsomal vesicles. The results demonstrate the existence of a separate intraluminal pyridine nucleotide pool in the hepatic endoplasmic reticulum and a close cooperation between 11betaHSD1 and H6PDH based on their co-localization and the mutual generation of cofactors for each other.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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11-beta-Hydroxysteroid Dehydrogenase Type 1 / analysis
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11-beta-Hydroxysteroid Dehydrogenase Type 1 / metabolism*
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Animals
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Carbohydrate Dehydrogenases / analysis
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Carbohydrate Dehydrogenases / metabolism*
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Endoplasmic Reticulum / chemistry*
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Endoplasmic Reticulum / enzymology
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Endoplasmic Reticulum / metabolism*
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Male
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Microsomes, Liver / enzymology
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Microsomes, Liver / metabolism
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Pyridines / analysis*
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Rats
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Rats, Wistar
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Transport Vesicles / enzymology
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Transport Vesicles / metabolism
Substances
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Pyridines
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Carbohydrate Dehydrogenases
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galactose-6-phosphate dehydrogenase
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11-beta-Hydroxysteroid Dehydrogenase Type 1
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pyridine