Isolation and characterization of the MHC linked beta-type proteasome subunit MC13 cDNA

FEBS Lett. 1992 May 11;302(2):121-5. doi: 10.1016/0014-5793(92)80420-l.

Abstract

We have cloned and analysed the second mouse MHC-linked proteasome subunit, designated MC13, which appears to be homologous to the human RING10 proteasome protein. The isolated cDNA has an ORF encoding a protein of 276 amino acids with a molecular weight of ca. 30 kDa. Sequence alignment reveals that the subunit MC13 and several other mammalian proteasome subunits are encoded by a second proteasome gene family. This second gene family encodes subunits of the beta-type, reveals striking sequence similarities with the beta-subunit of archaebacterial proteasomes and is related to, but distinct from, the genes encoding the so-called alpha-type subunits.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Archaea / chemistry
  • Base Sequence
  • Cloning, Molecular
  • Cysteine Endopeptidases / chemistry
  • Cysteine Endopeptidases / genetics*
  • DNA / chemistry
  • DNA / isolation & purification*
  • Humans
  • Macromolecular Substances
  • Major Histocompatibility Complex / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Weight
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics*
  • Proteasome Endopeptidase Complex
  • Sequence Homology, Nucleic Acid

Substances

  • Macromolecular Substances
  • Multienzyme Complexes
  • DNA
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex

Associated data

  • GENBANK/S86390
  • GENBANK/S86392
  • GENBANK/S90294
  • GENBANK/S90296
  • GENBANK/S90298
  • GENBANK/X63568
  • GENBANK/X63569
  • GENBANK/X64449
  • GENBANK/X65503
  • GENBANK/X65504