Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A

Vaccine. 2006 Mar 15;24(12):2110-9. doi: 10.1016/j.vaccine.2005.11.026. Epub 2005 Nov 28.

Abstract

In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4+ and CD8+ T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Antigens, Bacterial / metabolism
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / immunology*
  • Dendritic Cells / immunology*
  • Epitopes, T-Lymphocyte / immunology*
  • Immunization
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium tuberculosis / immunology*
  • Retroviridae / genetics
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transduction, Genetic

Substances

  • Antigens, Bacterial
  • Bacterial Vaccines
  • Epitopes, T-Lymphocyte
  • antigen 85, Mycobacterium bovis