Abstract
The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Helminth / genetics
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Antigens, Helminth / immunology
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DNA, Circular / genetics
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DNA, Helminth / genetics
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Gene Expression Profiling / methods
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Genes, Helminth / genetics*
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Helminth Proteins / genetics
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Larva / genetics
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Life Cycle Stages / genetics
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Lung / immunology*
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Membrane Proteins / genetics
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Mice
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Mice, Inbred Strains
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Oligonucleotide Array Sequence Analysis / methods*
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RNA, Helminth / genetics
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Schistosoma mansoni / genetics*
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Schistosoma mansoni / immunology
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Transcription, Genetic / genetics
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Up-Regulation / genetics
Substances
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Antigens, Helminth
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DNA, Circular
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DNA, Helminth
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Helminth Proteins
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Membrane Proteins
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RNA, Helminth