Promoter trapping in Magnaporthe grisea

J Zhejiang Univ Sci B. 2006 Jan;7(1):28-33. doi: 10.1631/jzus.2006.B0028.

Abstract

Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal / genetics
  • Genes, Reporter / genetics*
  • Genetic Vectors / genetics
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Magnaporthe / genetics*
  • Magnaporthe / metabolism*
  • Promoter Regions, Genetic / genetics*
  • Protein Engineering / methods*
  • Recombinant Proteins / metabolism*

Substances

  • Fungal Proteins
  • Recombinant Proteins
  • Green Fluorescent Proteins