Abstract
Enterococcus faecium is a frequently antibiotic-resistant opportunistic pathogen that is commonly recovered from hospitalized patients. The genetic organization of the dnaK operon was analyzed and was shown to consist of at least four heat shock genes, hrcA-grpE-dnaK-dnaJ. The dnaK/J intergenic region was 140 bp shorter than in E. faecalis. The dnaK operon was expressed from a putative sigma(A)-type promoter (PhrcA) upstream of the hrcA start codon and was preceded by two conserved CIRCE sequences. Northern hybridization revealed the presence of multiple mRNAs in the dnaK operon. Conversely, the groE operon was transcribed as a single mRNA. Induction of dnaK and groEL genes occurred in response to either heat shock or exposure to other stress agents.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Base Sequence
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Chaperonin 60 / genetics
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DNA, Intergenic
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Enterococcus faecalis / genetics
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Enterococcus faecium / genetics*
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Gene Expression Regulation
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Gene Expression Regulation, Bacterial
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Gene Order
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HSP40 Heat-Shock Proteins / genetics
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Heat-Shock Proteins / genetics*
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Hot Temperature
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Molecular Chaperones / genetics*
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Molecular Sequence Data
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Operon / genetics*
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Osmotic Pressure
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Oxidative Stress
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Promoter Regions, Genetic
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RNA, Bacterial / analysis
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RNA, Messenger / analysis
Substances
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Bacterial Proteins
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Chaperonin 60
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DNA, Intergenic
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GrpE protein, Bacteria
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HSP40 Heat-Shock Proteins
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Heat-Shock Proteins
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Molecular Chaperones
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RNA, Bacterial
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RNA, Messenger