Abstract
In a patient with chronic active Epstein-Barr virus infection associated with vasculitis and fulminant CD4+ T cell lymphoproliferative disorder, we probed the peripheral blood mononuclear cells (PBMC) for the presence of an EBV-specific T cell repertoire and tested the possible relationship between the lymphocytic infiltrate and the EBV-specific T cell response. Our results give credence to the presence of an apparently normal EBV-specific memory T cell response after in vitro reactivation of the patient's PBMC with autologous infected B lymphoblastoid cell lines. In keeping with the characterization of the vasculitis, certain T cell subsets were detected after expansion of skin lesion-infiltrating lymphocytes and were found to be infected with EBV. These particular T cell expansions were neither the effectors nor the targets of the in vitro reactivated EBV-specific T cells, thus excluding a simple relationship between EBV, the skin lesions, and the T cell expansions frequently observed in these patients.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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CD8-Positive T-Lymphocytes / immunology
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COS Cells
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Cell Line
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Cell Separation
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Chlorocebus aethiops
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Chronic Disease
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Coculture Techniques
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Complementarity Determining Regions / genetics
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Cytotoxicity, Immunologic / immunology
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DNA, Viral / analysis
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Epitopes, T-Lymphocyte / genetics
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Epitopes, T-Lymphocyte / immunology*
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Epstein-Barr Virus Infections / complications
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Epstein-Barr Virus Infections / immunology*
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Genes, MHC Class I / genetics
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / immunology*
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Humans
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / immunology
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Lymphoproliferative Disorders / etiology
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Lymphoproliferative Disorders / immunology
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Male
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Skin / immunology
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Skin / pathology
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T-Lymphocyte Subsets / chemistry
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / virology
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T-Lymphocytes / chemistry
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T-Lymphocytes / immunology*
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T-Lymphocytes / virology
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T-Lymphocytes, Cytotoxic / cytology
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism
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Transfection
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Tumor Necrosis Factor-alpha / metabolism
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Vasculitis / etiology
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Vasculitis / immunology
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Viral Proteins / genetics
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Viral Proteins / immunology
Substances
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Complementarity Determining Regions
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DNA, Viral
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Epitopes, T-Lymphocyte
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Peptide Fragments
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Receptors, Antigen, T-Cell, alpha-beta
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Tumor Necrosis Factor-alpha
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Viral Proteins