Abstract
RNA interference (RNAi) has been employed as an effective tool for studying gene functions. Here, we have demonstrated that different short hairpin RNAs (shRNAs) can specifically knock-down the transcription of STAT6, resulting in the inhibition of cell proliferation, G1/S phase arrest and apoptotic death in colon cancer HT-29 cells. These findings suggest that Stat6 signaling pathway plays a role in the regulation of cell proliferation and apoptosis in colon cancer cells, which promises further investigation for targeted cancer therapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / genetics*
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Apoptosis / physiology
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Cell Cycle / genetics
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Cell Cycle / physiology
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Cell Proliferation*
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Cell Survival / genetics
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Cell Survival / physiology
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Colonic Neoplasms / genetics
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Colonic Neoplasms / pathology
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Flow Cytometry
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HT29 Cells
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Humans
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Plasmids / genetics
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RNA Interference*
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RNA, Double-Stranded / genetics*
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STAT6 Transcription Factor / genetics*
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STAT6 Transcription Factor / physiology
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Transfection
Substances
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RNA, Double-Stranded
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STAT6 Transcription Factor