Abstract
A genome-independent microarray and new statistical techniques were used to genotype Bacillus strains and quantitatively compare DNA fingerprints with the known taxonomy of the genus. A synthetic DNA standard was used to understand process level variability and lead to recommended standard operating procedures for microbial forensics and clinical diagnostics.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus / classification
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Bacillus / genetics
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Bacillus / isolation & purification*
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Bacterial Typing Techniques*
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DNA Fingerprinting / methods
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Genome, Bacterial
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Nucleic Acid Hybridization
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Oligonucleotide Array Sequence Analysis / methods*
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Phylogeny