Abstract
A methylene chloride soluble fraction of the fruits of Cudrania tricuspidata significantly inhibited the mouse brain monoamine oxidase (MAO). Three known prenylated isoflavones were isolated and identified by activity-guided fractionation. Gancaonin A (1), 4'-O-methylalpinumisoflavone (2), and alpinumisoflavone (3) inhibited MAO activity in a concentration-dependent manner with IC50 values of 19.4, 23.9, and 25.8 microM, respectively. Of these, gancaonin A (1) showed a selective and potent inhibitory effect against MAO-B (IC50 0.8 microM) than MAO-A (IC50 >800 microM). The kinetic analysis using Lineweaver-Burk plots indicated that gancaonin A (1) competitively inhibited MAO-B.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain / enzymology
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Dose-Response Relationship, Drug
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Fruit / chemistry*
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Isoflavones / chemistry
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Isoflavones / isolation & purification
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Isoflavones / pharmacology
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Kinetics
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Maclura*
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Methylene Chloride / chemistry
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Mice
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Monoamine Oxidase / metabolism
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Monoamine Oxidase Inhibitors / chemistry
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Monoamine Oxidase Inhibitors / isolation & purification*
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Monoamine Oxidase Inhibitors / pharmacology
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plants, Medicinal / chemistry
Substances
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4'-O-methylalpinumisoflavone
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Isoflavones
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Monoamine Oxidase Inhibitors
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Plant Extracts
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gancaonin A
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Methylene Chloride
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alpinumisoflavone
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Monoamine Oxidase