Abstract
In this study, ethanolic extracts from 18 seaweed variants were assessed for hepatoprotective activity against tacrine-induced cytotoxicity in Hep G2 cells. Only one of these, Ecklonia stolonifera Okamura (Laminariaceae), a member of the brown algae, exhibited promising hepatoprotective activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction obtained from the ethanolic extract of E. stolonifera, resulted in the isolation of several phlorotannins [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 2 and 4 were determined to protect Hep G2 cells against the cytotoxic effects of tacrine, with EC50 values of 62.0 and 79.2 microg/mL, respectively. Silybin, a well characterized hepatoprotective agent, was used as a positive control, and exhibited an EC50 value of 50.0 microg/mL. It has been suggested that the phlorotannins derived from marine brown algae might prove useful sources in the development of novel hepatoprotective agents.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzofurans / chemistry
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Benzofurans / isolation & purification
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Benzofurans / pharmacology
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Cell Line, Tumor
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Cell Survival / drug effects
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Chemical Fractionation / methods
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Dioxanes / chemistry
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Dioxanes / isolation & purification
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Dioxanes / pharmacology
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Dioxins / chemistry
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Dioxins / isolation & purification
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Dioxins / pharmacology
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Ethanol / chemistry
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Heterocyclic Compounds, 4 or More Rings / chemistry
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Heterocyclic Compounds, 4 or More Rings / isolation & purification
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Heterocyclic Compounds, 4 or More Rings / pharmacology
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Humans
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Inhibitory Concentration 50
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Liver / drug effects*
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Liver / pathology
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Phaeophyceae / chemistry*
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Phloroglucinol / chemistry
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Phloroglucinol / pharmacology
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Protective Agents / chemistry
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Protective Agents / isolation & purification
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Protective Agents / pharmacology*
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Silybin
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Silymarin
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Tacrine / pharmacology
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Tannins / chemistry
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Tannins / isolation & purification
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Tannins / pharmacology
Substances
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Benzofurans
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Dioxanes
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Dioxins
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Heterocyclic Compounds, 4 or More Rings
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Protective Agents
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Silymarin
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Tannins
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eckstolonol
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phlorofucofuroeckol A
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Ethanol
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Silybin
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Tacrine
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Phloroglucinol