Effect of interleukin-1 beta on gene expressions and functions of fibroblastic cells derived from human periodontal ligament

J Periodontal Res. 1992 Jul;27(4 Pt 1):250-5. doi: 10.1111/j.1600-0765.1992.tb01675.x.

Abstract

The present study shows the effect of interleukin-1 beta (IL-1 beta) on some gene expressions and functions of fibroblastic cells (HPLF) derived from human periodontal ligament. HPLF were used at passages number 5 to 10. IL-1 beta increased DNA synthesis in both a dose- and an incubation time-dependent manner. IL-1 beta in combination with tumor-necrosis factor alpha or transforming growth factor beta synergistically stimulated the DNA synthesis in the cells. Since many studies have shown that the c-myc oncogene is involved in cell proliferation and differentiation, the effect of IL-1 beta on c-myc messenger RNA (mRNA) level in HPLF was examined. IL-1 beta induced a marked c-myc mRNA level in the cells at 90 minutes after initiation of the cytokine treatment. On the other hand, IL-1 beta significantly inhibited alkaline phosphatase (ALP) activity of the cells in a dose-dependent manner. Also an inhibitory effect was observed on the liver/bone/kidney ALP mRNA level of the cells, and this inhibition by IL-1 beta was dose- and incubation time-dependent. These results suggest that IL-1 beta is a regulatory cytokine involved in the regeneration of the human periodontal ligament.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / antagonists & inhibitors
  • Blotting, Northern
  • DNA / biosynthesis
  • Fibroblasts / drug effects*
  • Fibroblasts / enzymology
  • Fibroblasts / metabolism
  • Gene Expression Regulation / drug effects*
  • Gene Expression Regulation / physiology
  • Genes, myc / drug effects*
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-1 / physiology*
  • Periodontal Ligament / drug effects
  • Periodontal Ligament / metabolism*
  • Periodontal Ligament / physiology
  • RNA, Messenger / analysis
  • Recombinant Proteins / pharmacology
  • Regeneration / drug effects
  • Regeneration / physiology*

Substances

  • Interleukin-1
  • RNA, Messenger
  • Recombinant Proteins
  • DNA
  • Alkaline Phosphatase