Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli

Carbohydr Res. 2006 Mar 20;341(4):485-91. doi: 10.1016/j.carres.2005.11.032. Epub 2006 Jan 9.

Abstract

A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an alpha-(1-->6)-d-glucan, with a weight-average molecular weight of about 14,000Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aconitum / chemistry*
  • Animals
  • Antibody Formation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Drugs, Chinese Herbal / chemistry
  • Female
  • Lymphocytes / cytology
  • Lymphocytes / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Plant Roots / chemistry
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology
  • Spleen / cytology
  • Spleen / drug effects

Substances

  • Drugs, Chinese Herbal
  • FPS-1 polysaccharide
  • Polysaccharides