Abstract
Neuroblastoma (NBL), derived from the sympathetic precursor cells, is one of the most common pediatric solid tumors. The expression of N-acetylglucosaminyltransferase V and IX (GnT-V and GnT-IX) mRNA in 126 primary NBLs were quantitatively analyzed and higher expression levels of GnT-V were found to be associated with favorable stages (1, 2 and 4s). Conversely, the downregulation of GnT-V expression by small interfering RNA resulted in a decrease in the susceptibility to cell apoptosis induced by retinoic acid in NBL cells accompanied by morphological change. These results suggest that GnT-V is associated with prognosis by modulating the sensitivity of NBLs to apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / metabolism
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Apoptosis / physiology*
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Cell Line, Tumor
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Cell Survival
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Child
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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Humans
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Isoenzymes / metabolism*
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N-Acetylglucosaminyltransferases / genetics
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N-Acetylglucosaminyltransferases / metabolism*
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Neuroblastoma / enzymology*
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Neuroblastoma / genetics
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Neuroblastoma / pathology
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Tretinoin / metabolism
Substances
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Antineoplastic Agents
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Isoenzymes
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RNA, Small Interfering
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Tretinoin
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N-Acetylglucosaminyltransferases
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alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase