Tudor, MBT and chromo domains gauge the degree of lysine methylation

EMBO Rep. 2006 Apr;7(4):397-403. doi: 10.1038/sj.embor.7400625. Epub 2006 Jan 13.

Abstract

The post-translational modification of histones regulates many cellular processes, including transcription, replication and DNA repair. A large number of combinations of post-translational modifications are possible. This cipher is referred to as the histone code. Many of the enzymes that lay down this code have been identified. However, so far, few code-reading proteins have been identified. Here, we describe a protein-array approach for identifying methyl-specific interacting proteins. We found that not only chromo domains but also tudor and MBT domains bind to methylated peptides from the amino-terminal tails of histones H3 and H4. Binding specificity observed on the protein-domain microarray was corroborated using peptide pull-downs, surface plasma resonance and far western blotting. Thus, our studies expose tudor and MBT domains as new classes of methyl-lysine-binding protein modules, and also demonstrates that protein-domain microarrays are powerful tools for the identification of new domain types that recognize histone modifications.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Histones / metabolism
  • Lysine / genetics
  • Lysine / metabolism*
  • Methylation
  • Protein Array Analysis
  • Protein Binding
  • Protein Structure, Tertiary

Substances

  • Histones
  • Lysine