Abstract
In this study, we developed a simple and sensitive assay in mice for a challenge experiment by using a recombinant vaccinia virus dual-expressing antigen (HIV Env gp160) and firefly luciferase. This assay can detect the vaccine effect at real-time in vivo by using a small amount of mouse serum. The luciferase activity in mouse serum was in agreement with the viral titer in the ovary. This assay would be applicable as a challenge model for infectious diseases.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Vaccines / immunology
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Animals
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Disease Models, Animal*
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Female
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Genes, Reporter
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HIV / genetics*
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HIV Envelope Protein gp160 / genetics*
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HIV Envelope Protein gp160 / immunology
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HIV Infections*
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Luciferases / blood
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Luciferases / genetics*
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Mice
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Mice, Inbred BALB C
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Ovary / virology
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Sensitivity and Specificity
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Serum / virology
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Staining and Labeling / methods
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Vaccinia virus / genetics*
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Vaccinia virus / physiology
Substances
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AIDS Vaccines
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HIV Envelope Protein gp160
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Luciferases