Abstract
Objective:
To study the effect of Kanglaite injection on cyclooxygenase activity in lung carcinoma A549 cells.
Methods:
The expression of mRNA of COX-1 and COX-2 were measured by RT-PCR. The expression of COX-2 protein was measured by Western-blot.
Result:
Kanglaite injection could selectively decreased COX-2 mRNA expression and protein expression while COX-1 mRNA expression was unchanged.
Conclusion:
Kanglaite injection is selectively inhibitory agent of COX-2, and possess inhibitory effects on cyclooxygenase 2.
MeSH terms
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Antineoplastic Agents, Phytogenic / administration & dosage
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Carcinoma, Non-Small-Cell Lung / enzymology
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Carcinoma, Non-Small-Cell Lung / pathology
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Cell Line, Tumor
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Coix* / chemistry
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Cyclooxygenase 1 / biosynthesis
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Cyclooxygenase 1 / genetics
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Cyclooxygenase 2 / biosynthesis*
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Cyclooxygenase 2 / genetics
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Drugs, Chinese Herbal / administration & dosage
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Drugs, Chinese Herbal / isolation & purification
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Drugs, Chinese Herbal / pharmacology
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Gene Expression Regulation, Neoplastic
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Humans
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Injections
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Lung Neoplasms / enzymology*
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Lung Neoplasms / pathology
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Plant Oils / administration & dosage
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Plant Oils / isolation & purification
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Plant Oils / pharmacology*
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Plants, Medicinal / chemistry
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Seeds / chemistry
Substances
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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Plant Oils
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RNA, Messenger
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Cyclooxygenase 1
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Cyclooxygenase 2