Catheter-related bloodstream infections are common in hospitalized patients. Peripheral venous catheters (PVCs) are the most frequently used vascular access devices with usually low reported infection rates. We conducted a study to find the rate of PVC-related infections using semiquantitative (roll plate) and quantitative catheter culture techniques. We found significant growth in 9.5% of the PVCs by quantitative culture, which was predictive of a BSI in 43% of cases, which seems to be the major limitation of the method. We conclude that Gram stain is a fast and reliable diagnostic tool for rapid detection of PVC infection.