Abstract
The effects of a novel nutrient formulation Epican Forte (EF) were evaluated on proliferation and induction of apoptosis using non-cytotoxic concentrations against HTLV-1 positive (HuT-102 & C91-PL) and negative (CEM & Jurkat) cells. EF showed anti-proliferative effect as determined by MTT assay and TGF mRNA protein expression using RT-PCR. EF resulted in the down-regulation of TGF-alpha and an up-regulation in TGF-beta2. EF caused a significant increase in apoptotic cells in the preG1 phase. These results were confirmed using Cell Death ELISA and Annexin V-FITC. Induction of apoptosis was caused by an up-regulation of p53, p21 and Bax protein levels and a down-regulation of Bcl-2alpha protein expression level.
MeSH terms
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Acetylcysteine / pharmacology*
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects*
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Arginine / pharmacology*
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Ascorbic Acid / pharmacology*
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Catechin / analogs & derivatives*
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Catechin / pharmacology
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Cell Cycle / drug effects
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Copper / pharmacology*
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DNA / analysis
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Dietary Supplements
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Enzyme-Linked Immunosorbent Assay / methods
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Flow Cytometry
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Gene Expression Profiling
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Human T-lymphotropic virus 1 / drug effects*
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Human T-lymphotropic virus 1 / isolation & purification
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Humans
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Lysine / pharmacology*
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Manganese / pharmacology*
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Proline / pharmacology*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Selenium / pharmacology*
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Sensitivity and Specificity
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Structure-Activity Relationship
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T-Lymphocytes / drug effects*
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T-Lymphocytes / virology
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Transforming Growth Factors / genetics
Substances
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Antineoplastic Agents
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Manganese
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Transforming Growth Factors
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Copper
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Catechin
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DNA
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Arginine
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Proline
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epigallocatechin gallate
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Selenium
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Lysine
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Ascorbic Acid
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Acetylcysteine