Abstract
Cattle were vaccinated with an adenovirus expressing the mycobacterial antigen 85A (rAd85A), with Mycobacterium bovis BCG followed by rAd85A heterologous boosting, or with rAd85A followed by BCG boosting. BCG/rAd85A resulted in the highest direct gamma interferon responses. Cultured enzyme-linked immunospot assay analysis demonstrated that memory responses were induced by all three protocols but were strongest after BCG/rAd85A and rAd85A/BCG vaccination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics*
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Adenoviridae / immunology
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Animals
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Antigens, Bacterial / administration & dosage*
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Antigens, Bacterial / genetics
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Antigens, Bacterial / immunology*
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BCG Vaccine / administration & dosage*
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BCG Vaccine / immunology*
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Cattle
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Immunization Schedule
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Immunization, Secondary
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Interferon-gamma / biosynthesis*
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Leukocytes, Mononuclear / immunology
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Lymphocyte Activation
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Mycobacterium bovis / immunology*
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Tuberculosis, Bovine / immunology
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Tuberculosis, Bovine / prevention & control*
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Vaccination
Substances
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Antigens, Bacterial
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BCG Vaccine
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antigen 85, Mycobacterium bovis
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Interferon-gamma