Abstract
Investigation of phenolic patterns from the stems of Dendrobium chrysanthum by HPLC-PDA-MS has led to the isolation of a new phenanthrene derivative with a spirolactone ring, dendrochrysanene (1), that proved to suppress the mRNA level of TNF-alpha, IL8, IL10, and iNOS in murine peritoneal macrophages. The structure of 1 was characterized on the basis of various NMR (1H, 13C, 1H-1H COSY, HMQC, and HMBC), mass spectrometry, and X-ray crystal diffraction data.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / pharmacology
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Cells, Cultured
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Crystallography, X-Ray
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Dendrobium / chemistry*
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Drugs, Chinese Herbal / chemistry
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Interleukins / metabolism
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Macrophages, Peritoneal / drug effects
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Male
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Mice
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Mice, Inbred C57BL
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Phenanthrenes / chemistry*
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Phenanthrenes / metabolism
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Phenanthrenes / pharmacology*
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Spiro Compounds / chemistry*
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Spiro Compounds / metabolism
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Spiro Compounds / pharmacology*
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Spironolactone / chemistry*
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Tumor Necrosis Factors / metabolism
Substances
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Anti-Inflammatory Agents
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Drugs, Chinese Herbal
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Interleukins
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Phenanthrenes
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Spiro Compounds
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Tumor Necrosis Factors
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dendrochrysanene
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Spironolactone