Abstract
An increased level of cytokine interleukin-1 (IL-1) has been detected around the site of stroke. However, the effect of IL-1beta on the basilar artery has received little attention. We evaluated the effects of IL-1beta on the contractile response of rat isolated basilar artery by measuring isometric tension change. IL-1beta (10 ng/ml) and phenylephrine (0.1 nM) markedly enhanced U46619 (30 and 100 nM)-induced basilar artery contraction. The IL-1beta-mediated potentiation was partly suppressed by zinc protoporphyrin (3 microM) and was abolished by tetrodotoxin (TTX, 100 nM), (-)-perillic acid (1 microM), PD98059 (0.3 microM), SB203580 (1 microM) and prazosin (1 microM). Our data suggest that IL-1beta (10 ng/ml) causes an enhancement of U46619-mediated basilar artery contraction that probably involves TTX-sensitive neuronal release of an alpha1-adrenoceptor agonist and activation of p42/p44 and p38 mitogen-activated protein kinases/p21(ras) pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
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Adrenergic alpha-Antagonists / pharmacology
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Animals
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Basilar Artery / drug effects*
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Basilar Artery / physiology
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Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
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Cyclohexenes
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Flavonoids / pharmacology
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Imidazoles / pharmacology
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In Vitro Techniques
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Interleukin-1 / pharmacology*
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Male
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Monoterpenes / pharmacology
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Phenylephrine / pharmacology
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Prazosin / pharmacology
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Protoporphyrins / pharmacology
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Pyridines / pharmacology
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Rats
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Rats, Sprague-Dawley
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Tetrodotoxin / pharmacology
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Vasoconstriction / drug effects*
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Vasoconstrictor Agents / pharmacology
Substances
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Adrenergic alpha-Antagonists
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Cyclohexenes
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Enzyme Inhibitors
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Flavonoids
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Imidazoles
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Interleukin-1
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Monoterpenes
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Protoporphyrins
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Pyridines
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Vasoconstrictor Agents
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zinc protoporphyrin
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Phenylephrine
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Tetrodotoxin
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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perillic acid
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Calcium-Calmodulin-Dependent Protein Kinases
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SB 203580
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2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
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Prazosin