Expression of a truncated protein-tyrosine phosphatase mRNA in human lung

FEBS Lett. 1991 May 6;282(2):285-8. doi: 10.1016/0014-5793(91)80496-p.

Abstract

Protein-tyrosine phosphatases (PTPases) are becoming an important family of enzymes that might regulate key events in cell growth and transformation. While isolating a new member of this family via amplification of human lung cDNA by the polymerase chain reaction, we found a clone identical to but truncated at the 3'-end of the coding region of human PTPase beta (HPTP beta) mRNA. This difference in sequence is situated in the most conserved part of the catalytic domain of the enzyme. The expression level of the truncated form of HPTP beta mRNA in human lung was lower than its normal form.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Humans
  • Lung / enzymology*
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Phosphoprotein Phosphatases / genetics*
  • Polymerase Chain Reaction
  • Protein Tyrosine Phosphatases

Substances

  • Oligonucleotides
  • DNA
  • Phosphoprotein Phosphatases
  • Protein Tyrosine Phosphatases