Etiologic diagnosis of adult bacterial pneumonia by culture and PCR applied to respiratory tract samples

J Clin Microbiol. 2006 Feb;44(2):643-5. doi: 10.1128/JCM.44.2.643-645.2006.

Abstract

Respiratory culture and multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, and Chlamydophila pneumoniae were applied to sputum, nasopharyngeal swabs, and nasopharyngeal aspirates from 235 adult patients with community-acquired pneumonia and 113 controls. Both culture and multiplex PCR performed well with the different samples and appear to be useful as diagnostic tools.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacteriological Techniques
  • Chlamydophila pneumoniae / classification
  • Chlamydophila pneumoniae / genetics
  • Chlamydophila pneumoniae / isolation & purification
  • Community-Acquired Infections / diagnosis
  • Community-Acquired Infections / microbiology
  • Culture Media*
  • DNA, Bacterial / analysis
  • Haemophilus influenzae / classification
  • Haemophilus influenzae / genetics
  • Haemophilus influenzae / isolation & purification
  • Humans
  • Middle Aged
  • Mycoplasma pneumoniae / classification
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification
  • Pneumonia, Bacterial / diagnosis*
  • Pneumonia, Bacterial / microbiology*
  • Polymerase Chain Reaction / methods*
  • Respiratory System / microbiology
  • Sensitivity and Specificity
  • Streptococcus pneumoniae / classification
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification

Substances

  • Culture Media
  • DNA, Bacterial