Abstract
Cytoplasmic phospholipase A2 (cPLA2) has a key role in prostaglandin production. The role of cPLA2 in intestinal tumorigenesis has been suggested, however, contradictory data are found in the literature. We evaluated cPLA2 and cyclooxygenase-2 (COX-2) protein expression in 65 colon carcinomas by immunohistochemistry, and in eight colorectal cancer cell lines by Western Blot. PGE2 production was evaluated by enzyme-immunoassay in the cell lines. We demonstrate that cPLA2 is overexpressed in approximately 50% of colon cancers and cell lines. cPLA2 expression is correlated with COX-2 expression. Both cPLA2 and COX-2 expressions are important in regard to PGE2 production. Our data suggest that cPLA2 might be involved in colon tumor development.
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology*
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Blotting, Western
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Caco-2 Cells
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Cell Line, Tumor
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / metabolism
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Colorectal Neoplasms / pathology*
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Cyclooxygenase 2 / genetics*
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Cyclooxygenase 2 / metabolism
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Cytoplasm / enzymology
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Dinoprostone / biosynthesis*
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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HCT116 Cells
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HT29 Cells
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Humans
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Immunohistochemistry
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Microsatellite Instability
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Neoplasm Staging
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Phospholipases A / genetics*
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Phospholipases A / metabolism
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Phospholipases A2
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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RNA, Messenger
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Cyclooxygenase 2
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Phospholipases A
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Phospholipases A2
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Dinoprostone