Abstract
A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Affinity Labels
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Bacillus megaterium / enzymology*
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Bacillus megaterium / genetics*
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Bacterial Proteins / genetics
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Chromatography, Affinity
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Culture Media
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Gene Expression
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Genes, Bacterial
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Genetic Vectors
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Hexosyltransferases / biosynthesis*
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Hexosyltransferases / genetics*
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Hexosyltransferases / isolation & purification
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Limosilactobacillus reuteri / enzymology
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Limosilactobacillus reuteri / genetics
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Plasmids / genetics*
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Protein Sorting Signals / genetics
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
Substances
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Affinity Labels
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Bacterial Proteins
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Culture Media
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LipA protein, Bacteria
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Protein Sorting Signals
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Recombinant Fusion Proteins
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Hexosyltransferases
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levansucrase