Dendritic cells rapidly recruited into epithelial tissues via CCR6/CCL20 are responsible for CD8+ T cell crosspriming in vivo

Marie Le Borgne; Nathalie Etchart; Anne Goubier; Sergio A Lira; Jean Claude Sirard; Nico van Rooijen; Christophe Caux; Smina Aït-Yahia; Alain Vicari; Dominique Kaiserlian; Bertrand Dubois

doi:10.1016/j.immuni.2006.01.005

Dendritic cells rapidly recruited into epithelial tissues via CCR6/CCL20 are responsible for CD8+ T cell crosspriming in vivo

Immunity. 2006 Feb;24(2):191-201. doi: 10.1016/j.immuni.2006.01.005.

Authors

Marie Le Borgne¹, Nathalie Etchart, Anne Goubier, Sergio A Lira, Jean Claude Sirard, Nico van Rooijen, Christophe Caux, Smina Aït-Yahia, Alain Vicari, Dominique Kaiserlian, Bertrand Dubois

Affiliation

¹ INSERM U404 "Immunité et Vaccination", IFR128 BioSciences Lyon-Gerland, F-69365 Lyon, France.

PMID: 16473831
DOI: 10.1016/j.immuni.2006.01.005

Abstract

The nature of dendritic cell(s) (DC[s]) that conditions efficient in vivo priming of CD8+ CTL after immunization via epithelial tissues remains largely unknown. Here, we show that myeloid DCs rapidly recruited by adjuvants into the buccal mucosa or skin are essential for CD8+ T cell crosspriming. Recruitment of circulating DC precursors, including Gr1+ monocytes, precedes the sequential accumulation of CD11c+ MHC class II+ DCs in dermis and epithelium via a CCR6/CCL20-dependent mechanism. Remarkably, a defect in CCR6, local neutralization of CCL20, or depletion of monocytes prevents in vivo priming of CD8+ CTL against an innocuous protein antigen administered with adjuvant. In addition, transfer of CCR6-sufficient Gr1+ monocytes restores CD8+ T cell priming in CCR6( degrees / degrees ) mice via a direct Ag presentation mechanism. Thus, newly recruited DCs likely derived from circulating monocytes are responsible for efficient crosspriming of CD8+ CTL after mucosal or skin immunization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic
Animals
CD8-Positive T-Lymphocytes / immunology*
Cell Movement
Chemokine CCL20
Chemokines, CC / metabolism
Chemokines, CC / physiology*
Clodronic Acid / pharmacology
Dendritic Cells / immunology*
Dermis / metabolism
Dinitrofluorobenzene / pharmacology
Epithelium / immunology
Epithelium / metabolism
Female
H-2 Antigens / genetics
H-2 Antigens / physiology
Immunization
Langerhans Cells / physiology
Macrophage Inflammatory Proteins / metabolism
Macrophage Inflammatory Proteins / physiology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Mouth Mucosa / cytology
Mouth Mucosa / immunology
Mouth Mucosa / metabolism
Nucleoproteins / immunology
Receptors, CCR6
Receptors, Chemokine / genetics
Receptors, Chemokine / physiology*
Stem Cells / physiology

Substances

Adjuvants, Immunologic
CCL20 protein, human
CCR6 protein, human
Chemokine CCL20
Chemokines, CC
H-2 Antigens
Macrophage Inflammatory Proteins
Nucleoproteins
Receptors, CCR6
Receptors, Chemokine
Clodronic Acid
Dinitrofluorobenzene