Anti-cyclic citrullinated peptide antibodies in hepatitis C virus associated rheumatological manifestations and Sjogren's syndrome

Ann Rheum Dis. 2006 Mar;65(3):394-7. doi: 10.1136/ard.2005.038042.

Abstract

Objective: To investigate the diagnostic reliability of anti-CCP antibodies (anti-CCP Ab) in distinguishing hepatitis C virus (HCV) associated rheumatological manifestations and Sjögren's syndrome from rheumatoid arthritis.

Methods: 147 HCV infected patients (HCV RNA positive) were compared with 64 patients with definite rheumatoid arthritis in a retrospective study. Anti-CCP Ab were detected using the Immunoscan ELISA kit (second generation) and rheumatoid factor (RF) by the FIDIStrade mark Rheuma kit.

Results: Among the 147 HCV infected patients (77 women; mean (SD) age 58 (16) years), 77 (52%) had a mixed cryoglobulin (MC), 38 (26%) an MC associated systemic vasculitis, 35 (24%) arthralgia/arthritis, and seven (5%) definite Sjögren's syndrome. HCV infected patients with arthralgia were more often RF positive than those without arthralgia (54% v 27%; p = 0.003), but less often than patients with rheumatoid arthritis (54% v 81%; p = 0.009). Anti-CCP Ab were detected in only two HCV infected patients with arthralgia (5.7%), in none without arthralgia or with Sjögren's syndrome, and in 78% of patients with rheumatoid arthritis. With a specificity of 93.5% and a positive predictive value of 96% for rheumatoid arthritis, anti-CCP Ab were the most specific biological marker.

Conclusions: Anti-CCP antibodies are very rarely found in HCV infected patients with rheumatological manifestations or Sjögren's syndrome. They are reliable serological markers to distinguish these from patients with rheumatoid arthritis.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Arthritis, Infectious / diagnosis*
  • Arthritis, Rheumatoid / diagnosis*
  • Autoantibodies / blood
  • Biomarkers / blood
  • Diagnosis, Differential
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Hepatitis C / diagnosis*
  • Humans
  • Male
  • Middle Aged
  • Peptides, Cyclic / immunology*
  • Retrospective Studies
  • Rheumatoid Factor / blood
  • Sensitivity and Specificity
  • Sjogren's Syndrome / diagnosis*

Substances

  • Autoantibodies
  • Biomarkers
  • Peptides, Cyclic
  • cyclic citrullinated peptide
  • Rheumatoid Factor