A flow cytometric screening test for detergent-resistant surface antigens in monocytes

Cytometry A. 2006 Mar;69(3):192-5. doi: 10.1002/cyto.a.20238.

Abstract

Rafts resemble cholesterol- and glycosphingolipid-enriched, liquid-ordered plasma membrane microdomains, showing resistance to nonionic detergents, and are involved in various cellular processes. In the present study, we have tested surface antigens on resting and lipopolysaccharide (LPS)-stimulated human peripheral blood monocytes for their detergent resistance (i.e. raft-association), by flow cytometry. Constitutive (CD14, CD32, CD55), or LPS-induced (CD81) raft-association, and detergent solubility (i.e. exclusion of rafts) (CD71) of monocyte antigens in the presence of 0.01% Triton X-100 are clearly demonstrated. Flow cytometric detergent insolubility is a powerful tool for rapid screening the raft-association of monocyte antigens in a whole-blood assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Antigens, Surface / analysis*
  • Antigens, Surface / metabolism
  • CD55 Antigens / analysis
  • Cell Membrane / chemistry
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Detergents / chemistry*
  • Flow Cytometry / methods*
  • Humans
  • Kinetics
  • Lipopolysaccharide Receptors / analysis
  • Lipopolysaccharides / pharmacology
  • Membrane Microdomains / chemistry*
  • Membrane Microdomains / drug effects
  • Membrane Microdomains / metabolism
  • Monocytes / chemistry*
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Octoxynol / chemistry
  • Receptors, IgG / analysis
  • Receptors, Transferrin / analysis
  • Solubility
  • Tetraspanin 28

Substances

  • Antigens, CD
  • Antigens, Surface
  • CD55 Antigens
  • CD71 antigen
  • CD81 protein, human
  • Detergents
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Receptors, IgG
  • Receptors, Transferrin
  • Tetraspanin 28
  • Octoxynol