A novel gamma subunit of the GABAA receptor identified using the polymerase chain reaction

FEBS Lett. 1991 Jun 24;284(2):211-5. doi: 10.1016/0014-5793(91)80687-x.

Abstract

We have utilized a polymerase chain reaction (PCR) strategy to identify a novel subunit, gamma 3, of the GABAA receptor. The gamma 3 cDNA encodes a mature protein of 450 amino acids that contains structural features typically conserved among subunits of the GABAA receptor family. The gamma 3 subunit shares approximately 66% sequence identity with the gamma 2 subunit but only 38% and 29% with alpha 1 and beta 1 subunits, respectively. Localization of the gamma 3 mRNA indicates that it is widely distributed throughout the mouse brain in a pattern similar to that observed for mRNAs encoding the gamma 2 subunits.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain Chemistry
  • Macromolecular Substances
  • Male
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction*
  • RNA, Messenger / analysis
  • Receptors, GABA-A / chemistry*
  • Receptors, GABA-A / genetics
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution

Substances

  • Macromolecular Substances
  • RNA, Messenger
  • Receptors, GABA-A