Abstract
To elucidate the molecular biology of the hematopoietic stem cell, we have begun to isolate genes from murine cell populations enriched in stem cell activity. One such cDNA encodes a novel receptor tyrosine kinase, designated fetal liver kinase-2 or flk-2, which is related to the W locus gene product c-kit. Expression analyses suggest an extremely restricted distribution of flk-2. It is expressed in populations enriched for stem cells and primitive uncommitted progenitors, and is absent in populations containing more mature cells. Therefore, this receptor may be a key signal transducing component in the totipotent hematopoietic stem cell and its immediate self-renewing progeny.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cell Separation
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Cloning, Molecular
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DNA / genetics
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Gene Expression
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Hematopoiesis
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Hematopoietic Stem Cells / enzymology
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Hematopoietic Stem Cells / physiology*
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Liver / embryology
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Liver / physiology
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Mice
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Mice, Inbred Strains
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Molecular Sequence Data
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogene Proteins*
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RNA, Messenger / genetics
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Receptor Protein-Tyrosine Kinases*
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Receptors, Cell Surface / genetics*
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Thymus Gland / cytology
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Thymus Gland / physiology
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fms-Like Tyrosine Kinase 3
Substances
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Cell Surface
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DNA
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Flt3 protein, mouse
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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fms-Like Tyrosine Kinase 3
Associated data
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GENBANK/M59201
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GENBANK/M63700
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GENBANK/M63702
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GENBANK/M63718
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GENBANK/M64689
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GENBANK/S40354
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GENBANK/S63145
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GENBANK/S63146
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GENBANK/S63147
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GENBANK/S63148