Abstract
Expression of Leishmania donovani cyclin 1 (LdCyc1) mRNA during the cell cycle of promastigotes is S-phase specific. Here, we show that the LdCyc1 protein is periodically expressed and the activity of its associated kinase varies during the cell cycle in line with its expression pattern. In addition, we have shown that LdCRK3, homologous to CRK3 from L. mexicana, is the cognate Cdk partner of LdCyc1 and that the activity of the complex is inhibited specifically by heat stable factor(s) from the parasite.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CDC2 Protein Kinase / chemistry
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CDC2 Protein Kinase / metabolism*
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Cells, Cultured
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Cyclin A / metabolism
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Cyclin E / metabolism
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Cyclins / metabolism*
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Cyclins / physiology
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Gene Expression Profiling / methods
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HeLa Cells
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Humans
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Immunoblotting / methods
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Leishmania donovani / chemistry
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Leishmania donovani / cytology
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Leishmania donovani / physiology*
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Protein Kinases / analysis
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Protozoan Proteins / chemistry
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Protozoan Proteins / metabolism*
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Protozoan Proteins / physiology
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S Phase / physiology*
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S-Phase Kinase-Associated Proteins / physiology*
Substances
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Cyclin A
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Cyclin E
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Cyclins
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Protozoan Proteins
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S-Phase Kinase-Associated Proteins
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cyc1 protein, Paramecium tetraurelia
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Protein Kinases
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histone H1 kinase
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CDC2 Protein Kinase