Abstract
We report a novel Fourier-transform-based implementation of coherent anti-Stokes Raman scattering (CARS) microscopy. The method employs a single femtosecond laser source and a Michelson interferometer to create two pulse replicas that are fed into a scanning multiphoton microscope. By varying the time delay between the pulses, we time-resolve the CARS signal, permitting easy removal of the nonresonant background while providing high resolution, spectrally resolved images of CARS modes over the laser bandwidth (approximately 1500 cm(-1)). We demonstrate the method by imaging polystyrene beads in solvent.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Equipment Design
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Equipment Failure Analysis
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Fourier Analysis
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Image Enhancement / instrumentation*
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Image Enhancement / methods
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Image Interpretation, Computer-Assisted / methods*
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Information Storage and Retrieval / methods*
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Light
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Microscopy, Fluorescence, Multiphoton / instrumentation*
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Microscopy, Fluorescence, Multiphoton / methods
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Microscopy, Interference / instrumentation*
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Microscopy, Interference / methods
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Phantoms, Imaging
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Scattering, Radiation
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Signal Processing, Computer-Assisted
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Spectrum Analysis, Raman / instrumentation*
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Spectrum Analysis, Raman / methods
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Tomography, Optical Coherence / instrumentation*
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Tomography, Optical Coherence / methods