Abstract
Bone morphogenetic proteins (BMPs) initiate, promote, and maintain chondrogenesis and osteogenesis. We hypothesize that BMP-2 induces an osteogenic, and BMP-7 a chondrogenic phenotype in adipose tissue-derived mesenchymal stem cells (AT-MSCs). We compared the effects of a short 15min BMP-2 or BMP-7 (10ng/ml) treatment on osteogenic and chondrogenic differentiation of AT-MSCs. Gene expression was studied 4 and 14 days after BMP-treatment. At day 4 BMP-2, but not BMP-7, stimulated runx-2 and osteopontin gene expression, and at day 14 BMP-7 down-regulated expression of these genes. At day 4 BMP-2 and BMP-7 stimulated biglycan gene expression, which was down-regulated by BMP-7 at day 14. BMP-7 stimulated aggrecan gene expression at day 14. Our data indicate that BMP-2 treatment for 15min induces osteogenic differentiation, whereas BMP-7 stimulates a chondrogenic phenotype of AT-MSCs. Therefore, AT-MSCs triggered for only 15min with BMP-2 or BMP-7 provide a feasible tool for bone and cartilage tissue engineering.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / cytology*
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Aggrecans
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Alkaline Phosphatase / metabolism
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Animals
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Biglycan
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins / pharmacology*
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Cell Count
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Cell Differentiation / drug effects
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Cells, Cultured
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Chondrogenesis / drug effects*
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Chondroitin Sulfate Proteoglycans / genetics
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Core Binding Factor Alpha 1 Subunit / genetics
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Extracellular Matrix Proteins / genetics
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Gene Expression Regulation / drug effects
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Goats
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Humans
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Lectins, C-Type / genetics
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Osteogenesis / drug effects*
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Osteopontin
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Proteoglycans / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sialoglycoproteins / genetics
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Stem Cells / cytology*
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Stem Cells / drug effects*
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Stem Cells / metabolism
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Transforming Growth Factor beta / pharmacology*
Substances
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Aggrecans
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BGN protein, human
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BMP2 protein, human
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BMP7 protein, human
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Biglycan
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins
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Chondroitin Sulfate Proteoglycans
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Core Binding Factor Alpha 1 Subunit
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Extracellular Matrix Proteins
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Lectins, C-Type
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Proteoglycans
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RNA, Messenger
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SPP1 protein, human
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Sialoglycoproteins
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Transforming Growth Factor beta
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Osteopontin
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Alkaline Phosphatase