Abstract
In vitro immunization with HCV lipopeptide aa 20-44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-gamma secretion upon HCV-specific re-stimulation. This effect was related to significantly increased expression of TLR2 on DC-immunized CD4+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4+ T cells but not CD8+ T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic
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Adult
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Antigen Presentation*
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CD4-Positive T-Lymphocytes / immunology*
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CD8-Positive T-Lymphocytes / immunology
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Cell Proliferation
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Cysteine / analogs & derivatives
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Dendritic Cells / immunology*
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Female
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Granzymes
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Hepacivirus / immunology*
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Humans
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Immunization
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Interferon-gamma / biosynthesis
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Lipoproteins / immunology*
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Lymphocyte Subsets / immunology
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Male
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Middle Aged
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Serine Endopeptidases / analysis
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Toll-Like Receptor 2 / biosynthesis*
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Up-Regulation
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Viral Proteins / chemistry
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Viral Proteins / immunology*
Substances
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Adjuvants, Immunologic
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Lipoproteins
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TLR2 protein, human
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Toll-Like Receptor 2
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Viral Proteins
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Interferon-gamma
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tripalmitoyl cysteine
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GZMB protein, human
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Granzymes
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Serine Endopeptidases
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Cysteine