UV-resonance Raman spectroscopic study of human plasma of healthy donors and patients with thrombotic microangiopathy

Biopolymers. 2006 Jul;82(4):317-24. doi: 10.1002/bip.20489.

Abstract

Various diseases shift the composition of human plasma; hence, the relative quantification of plasma constituents offers the opportunity to use the dynamic and complex composition of plasma to gain information on novel diagnostic and prognostic factors. Since plasma contains, besides water, mostly proteins, UV-resonance Raman spectroscopy (UVRR) seems to be a suitable method for investigating plasma. With this method the signals of aromatic amino acids and proteins are selectively enhanced. In this study an UV-resonance Raman approach was used for the investigation of human plasma of healthy volunteers and patients with thrombotic microangiopathy. For comparison, selected plasma components were analyzed for a more detailed characterization of cryoprecipitates from human plasma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Plasma / chemistry*
  • Purpura, Thrombotic Thrombocytopenic / blood*
  • Spectrophotometry, Ultraviolet / methods
  • Spectrum Analysis, Raman / methods*
  • Tissue Donors*
  • Vibration