Abstract
We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained for subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays, cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays, enabling new classes of genetic assays based on cell imaging.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Gene Expression Profiling*
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Genes, Fungal / genetics
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Genome, Fungal
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Oligonucleotide Array Sequence Analysis
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Phenotype*
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Pheromones / genetics*
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Receptors, Mating Factor / genetics*
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Reproducibility of Results
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics
Substances
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Pheromones
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Receptors, Mating Factor
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Saccharomyces cerevisiae Proteins