Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction

Kristof Van Hecke; Koen Uytterhoeven; Sergey N Mikhailov; Piet Herdewijn; Luc Van Meervelt

doi:10.1107/S1744309105030381

Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt 10):953-5. doi: 10.1107/S1744309105030381. Epub 2005 Sep 30.

Authors

Kristof Van Hecke¹, Koen Uytterhoeven, Sergey N Mikhailov, Piet Herdewijn, Luc Van Meervelt

Affiliation

¹ Biomolecular Architecture, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, B-3001 Leuven (Heverlee), Belgium.

Abstract

Incorporation of a disaccharide nucleoside into double-stranded DNA can be considered as a chemical (non-enzymatic) alternative for site-specific cleavage of DNA. Crystals of the sequence d(CGCGAATT*CGCG), where * is an incorporated ribose, were obtained by hanging-drop vapour diffusion and diffracted to 2.6 A. The crystals belong to the orthorhombic space group P222(1), with unit-cell parameters a = 41.52, b = 57.63, c = 81.39 A, indicating a new crystal packing motif for an oligonucleotide dodecamer sequence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
DNA / chemistry*
Diffusion
Disaccharides*
Magnetic Resonance Spectroscopy
Models, Chemical
Models, Molecular
Mutagenesis, Site-Directed
Nucleic Acid Conformation*
Nucleosides / chemistry*
Oligonucleotides / chemistry
Ribose / chemistry
X-Ray Diffraction

Substances

Disaccharides
Nucleosides
Oligonucleotides
Ribose
DNA