Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt 2):94-6. doi: 10.1107/S1744309105040698. Epub 2006 Jan 27.

Abstract

Lectins with sugar-binding specificity are widely distributed in higher plants and various other species. The expression of rice lectin from Oryza sativa is up-regulated in the growing coleoptile when anaerobic stress persists. A rice lectin of molecular weight 15.2 kDa has been crystallized using the hanging-drop vapour-diffusion method. From the diffraction of the lectin crystals at 1.93 A resolution, the unit cell belongs to space group P3(1), with unit-cell parameters a = 98.58, b = 98.58, c = 44.72 A. Preliminary analysis indicates that there are two lectin molecules in an asymmetric unit with a large solvent content, 70.1%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobiosis / physiology
  • Crystallization
  • Crystallography, X-Ray
  • Oryza / growth & development
  • Oryza / metabolism
  • Oxidative Stress / physiology
  • Plant Lectins / biosynthesis
  • Plant Lectins / chemistry*
  • Plant Lectins / isolation & purification
  • Up-Regulation

Substances

  • Plant Lectins
  • rice lectin