Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase

Chem Commun (Camb). 2006 Mar 14:(10):1079-81. doi: 10.1039/b515763c. Epub 2006 Jan 24.

Abstract

Characterisation of recombinant Streptomyces spheroides NovW in vitro suggests that it is not a kinetically competent dual action dTDP-6-deoxy-d-xylo-4-hexulose 3,5-epimerase, but possesses only significant 3-epimerase activity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Epimerases / chemistry
  • Carbohydrate Epimerases / metabolism*
  • Catalysis
  • Kinetics
  • Models, Molecular
  • Molecular Structure
  • Novobiocin / biosynthesis
  • Novobiocin / chemistry
  • Solvents
  • Streptomyces / chemistry*
  • Streptomyces / enzymology*

Substances

  • Solvents
  • Novobiocin
  • Carbohydrate Epimerases