Abstract
Reverse transcription-polymerase chain reaction was used to develop a procedure with internal monitoring that may provide evidence for the absence of tick-borne encephalitis virus RNA in bioassays. The proposed procedure is useful in evaluating the safety and effectiveness of a tick-borne encephalitis vaccine.
MeSH terms
-
Animals
-
Cell Line
-
DNA Primers
-
Encephalitis Viruses, Tick-Borne / genetics
-
Encephalitis Viruses, Tick-Borne / isolation & purification*
-
Mice
-
Mice, Inbred BALB C
-
RNA, Viral / analysis*
-
Reverse Transcriptase Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity
-
Species Specificity
-
Viral Vaccines / standards
Substances
-
DNA Primers
-
RNA, Viral
-
Viral Vaccines