Abstract
Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Animals
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Cells, Cultured
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Cytoskeleton / chemistry
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Cytoskeleton / metabolism
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Growth Cones / chemistry
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Growth Cones / metabolism
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Growth Cones / physiology*
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Immunoprecipitation
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Mice
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Microfilament Proteins / analysis
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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Mutation
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurites / chemistry
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Neurites / physiology*
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Neurons / cytology
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Neurons / metabolism
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Neurons / physiology
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Nuclear Receptor Coactivator 3
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Oligodeoxyribonucleotides, Antisense / genetics
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Oligodeoxyribonucleotides, Antisense / pharmacology
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Protein Interaction Mapping
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Protein Structure, Tertiary
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Rats
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Transcription Factors / analysis
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation
Substances
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Actins
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Microfilament Proteins
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Nerve Tissue Proteins
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Oligodeoxyribonucleotides, Antisense
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Transcription Factors
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neurabin
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Nuclear Receptor Coactivator 3