Cysteine-free glutathione-S-tranferase as a tool for thiol-specific labeling of proteins

Biotechniques. 2006 Feb;40(2):145-6. doi: 10.2144/000112113.
No abstract available

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Chromatography, Gel
  • Circular Dichroism
  • Cysteine / chemistry*
  • Escherichia coli / genetics
  • Glutathione / metabolism
  • Glutathione Transferase / genetics
  • Glutathione Transferase / isolation & purification
  • Glutathione Transferase / metabolism*
  • Plasmids
  • Point Mutation
  • Polymerase Chain Reaction
  • Proteins / metabolism*
  • Sequence Analysis, DNA
  • Serine / metabolism
  • Spectrometry, Fluorescence
  • Sulfhydryl Compounds / metabolism*

Substances

  • Proteins
  • Sulfhydryl Compounds
  • Serine
  • Glutathione Transferase
  • Glutathione
  • Cysteine